Laboratory Safety Regulations
The material on this page is the same as in your Lab Manual, beginning on page 13.
1. Safety goggles and lab coats must be worn at all times. Corrective lenses, if needed, must be worn under the goggles. If a chemical should accidentally come in contact with the skin or eyes, flush immediately with large quantities of water and notify a lab instructor.
2. "Horseplay" will not be tolerated. Your laboratory instructor will ask you to leave the lab and assign you a grade of zero for that experiment if you persist in behavior that makes you a safety hazard to yourself or others.
3. In case of accident or injury, notify a lab instructor immediately.
4. Be familiar with the locations and use of showers, eye wash, fountains, first aid kits, fire blankets and fire exits.
5. Clothing must be appropriate for the laboratory. Shoes must completely cover the feet; SANDALS OR OTHER OPEN FOOTWEAR ARE NOT ACCEPTABLE. Long pants should be worn. Long hair and loose clothing must be confined or tied back.
6. No smoking, eating, or drinking allowed in the laboratory.
7. Do not attempt unauthorized experiments. Check with your lab TA first.
8. Never smell, taste, or eat any chemical. Treat all chemicals as poisonous. Never pipette by mouth.
9. To insert glass tubing or rods into stoppers or rubber hoses:
(a) Wrap cloth around glass and put on gloves.
(b) Make sure glass ends are smooth.
(c) Lubricate glass with glycerol or stopcock grease.
(d) Hold wrapped glass no more than 5 cm (2 inches) from end.
(e) Insert glass with easy twisting motion.
10. Dilute acids very carefully, always adding acid to water (not water to acid). Clean up spills immediately. Wear gloves when pouring concentrated acids or bases or when working with other hazardous substances.
11. Never point a test tube at yourself or anyone else, especially while heating. Use boiling chips to prevent "bumping" while heating. Do not used cracked glassware.
12. Before using any reagent, read the label carefully to be sure you have the right substance. Many distinctly different substances have similar names!
13. When pouring from a bottle, hold the label toward your palm to protect the label (and the hand of the next user) if reagent spills down the side of the bottle.
14. Use no more material than needed. Never return excess reagent to its original container. Dispose all waste in properly labeled containers as your TA instructs.
15. Before pouring a liquid into a buret or separatory funnel, be sure the stopcock is closed.
16. Use fume hoods for all work involving objectionable or dangerous gases or vapors.
17. Do not remove reagents from their shelves to your lab bench.
18. Many solvents are toxic and/or flammable. Do not inhale solvent fumes. Before pouring ether or other volatile organic solvents from a bottle, make sure there are no open flames anywhere near you. Before lighting a match, make sure no volatile solvents are being used nearby. Always close bottles after use. Clean up spills as soon as possible; your lab instructor will help you.
19. Do not throw matches, filter paper, or other refuse in the sinks; waste receptacles are provided under the hoods at the ends of the laboratories. If organic liquids are accidentally spilled into a sink, wash them down with water immediately. Place any broken glass in the special bucket for sharp objects.
20. Before leaving the lab, make sure that all gas and water connections on your bench are turned off, and clean up your workbench, hoods, sink, balance area, etc. All waste should be placed in the appropriate solid waste or liquid waste container in the last hood on right, on the back wall of the lab.